Institute of Technical Biocatalysis

Publications of Dr. Galunsky (retired 2014)

Type Year
Publications in journals 2010, 2009, 2008, 2006, 2005, 2004, 2003, 2002, 2001, 2000, -1999
patents -2008

Publications in journals


  • Ruslan Yuryev, Volker Kasche, Zoya Ignatova and Boris Galunsky (2010)

    Improved A. faecalis penicillin amidase mutant retains the thermodynamic and pH stability of the wild type enzyme. The Protein Journal (accepted).


  • Tishinov, K., Stambolieva, N., Nedkov, P., Galunsky, B. (2009)

    Sunflower seed aminopeptidase-catalyzed hydrolysis of Phe-Xaa dipeptides. Exploring the S1´ specificity of the enzyme.
    J.Plant Biochem Biotechnol 18, 237-239

  • Zhiryakova, D., Ivanov, I., Ilieva, S., Guncheva, M., Galunsky, B., Stambolieva, N. (2009)

    Are Ntn-hydrolases indeed with a single-amino-acid catalytic centre? Supporting amino acid residues at the active site of penicillin G acylase.
    FEBS J 276, 2589-2598.


  • Kiril Tishinov, Stanislav Bayramov, Peter Nedkov, Nikolona Stambolieva, Boris Galunsky (2009)

    A highly enantioselective aminopeptidase from sunflower seed – Kinetic studies, substrate mapping and application to biocatalytic transformations.
    J. Mol. Catal.B: Enzym. 59, 106-110.


  • Siwek, M., Noubar, A.M., Erdmann, R., Niemeyer, B., Galunsky, B. (2008)

    Application of mixed-mode Oasis MCX adsorbent for chromatographic separation of selenomethionine from Antarctic krill after enzymatic digestion.
    Chromatographia 67, 305-308


  • Siwek, M., Noubar, A.B., Bergmann, J., Niemeyer, B., Galunsky, B. (2006)

    Enhancement of enzymatic digestion of Antarctic krill and successive extraction of selenium organic compounds by ultrasound treatment.
    Anal. Bioanal. Chem. 384, 244-249.


  • Siwek, M., Noubar, A.B., Bergmann, J., Niemeyer, B., Galunsky, B. (2005)
    Enhancement of enzymatic digestion of Antarctic krill and successive extraction of selenium organic compounds by ultrasound treatment.
    Anal. Bioanal. Chem. in press.
  • Siwek, M., Galunsky, B., Niemeyer, B. (2005)
    Isolation of selenium organic species from Antarctic krill after enzymatic hydrolysis.
    Anal. Bioanal. Chem. 381, 737-741.


  • Bozhinova, D., Galunsky, B., Yueping, G., Franzreb, M., Köster, R., & Kasche, V. (2004)
    Evaluation of magnetic polymer micro-beads as carriers of for immobilised biocatalysts for selective and stereoselective transformations.
    Biotechnol. Lett. 26, 343-350.
  • Guncheva, M., Ivanov, I., Galunsky, B., Stambolieva, N., Kaneti, J. (2004)
    Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263.
    Eur. J. Biochem. 271, 2272-2279.


  • Kasche, V., Galunsky, B., Ignatova, Z. (2003)
    Fragments of pro-peptide activate mature penicillin amidase of Alcaligenes faecalis.
    Eur. J. Biochem. 270, 4721-4728.


  • Galunsky, B., Kasche, V. (2002)
    Determination of enantioselectivity for kinetically controlled condensations catalysed by amidases and peptidases.
    Adv. Synth. Catal. 344, 1115-1119.


  • Chickere, A.C., Galunsky, B., Schünemann, V., and Kasche, V. (2001)
    Stability of immobilised soybean lipoxygenases: infulence of coupling conditions on the ionisation state of the active site Fe.
    Enzyme Microb. Technol. 28, 168-175.


  • Chikere, A., Galunsky, B., Overmeyer, A., Brunner G., Kasche, V. (2000)
    Activity and stability of immobilised soybean lipoxigenase-1 in aqueous and supercritical carbon dioxide media.
    Biotechnol. Lett. 22, 1815-1821.
  • Galunsky, B., Lummer K. and Kasche, V. (2000)
    Comparative study of penicillin G amidases from different sources and hybrid isoenzymes.
    Chem. Monthly 131, 623-632.


  • Lummer, K., Riecks, A., Galunsky, B., and Kasche, V. (1999)
    pH-Dependence of penicillin amidase enantioselectivity for charged substrates.
    Biochem. Biophys. Acta 1433, 327-334.
  • A.Chikere, B.Galunsky, V.Kasche (1998)
    Soybean lipoxigenases: purification and stability of free and immobilised enzymes.
    In: A. Ballesteros, F.J. Plou, J.L. Iborra and P. Halling (Eds.) Stability and Stabilisation of Biocatalysts, Elsevier.
  • Z.Ignatova, S.Stoeva, B.Galunsky, C.Hörnle, A.Nurk, E.Piotraschke, W.Voelter, V.Kasche (1998)
    Proteolytic processing of penicillin amidase fropm Alcaligenes faecalis cloned in E.coli yields several active forms.
    Biotechnol. Lett. 20, 977-982.
  • N.Stambolieva, Z.Mincheva and B.Galunsky (1998)
    Kinetic comparison of penicillin amidase catalysed transfer of nonspecific and specific acyl moieties to 7-aminodeacetoxycephalosporanic acid.
    Biocat. Biotrans. 16, 225-232.
  • B.Galunsky, S.Ignatova, V.Kasche (1997)
    Temperature effects on S1- and S1�- enantioselectivity of &alpha-chymotrypsin.
    Biochim. Biophys. Acta 1343, 130-138.
  • Z.Mincheva, N.Stambolieva, K.Petrova, B.Galunsky (1996)
    Penicillin amidase catalysed preparative synthesis of cephem 7-(2-benzoxazolon-3-yl-acetamido)-desacetoxycephalosporanic acid using a non-specific polyethyleneglycol-modified acyl donor.
    Biotechnol. Techniques 10, 727-730.
  • V.Kasche, B.Galunsky, A.Nurk, E.Piotraschke, and A.Rieks (1996)
    Temperature and primary structure dependence of the stereoselectivity for penicillin amidases-enzymes with R-specific S1- and S-specific S1�-subsites.
    Biotechnol. Lett. 18, 445-460.
  • B.Böckle, B.Galunsky, R.Müller (1995)
    Characterization of a keratinolitic serine proteinase from Streptomyces pactum dsm 40530.
    Appl. Environ. Microbiol. 61, 3705-3710.
  • M.Klingeberg, B.Galunsky, C.Sjoholm, V.Kasche, G.Antranikian (1995)
    Purification and properties of highly thermostable, SDS resistant and stereospecific proteinase from Thermococcus stetteri.
    Appl. Environ. Microbiol. 61, 3098-3104.
  • V.Kasche, B.Galunsky (1995)
    Enzyme catalysed biotransformations in aqueous two-phase systems with precipitated substrate and/or product.
    Biotechnol. Bioeng. 45, 261-267.
  • B.Galunsky, R.-C. Schlothauer, B.Böckle, V.Kasche (1994)
    Direct spectrophotometric measurement of enzyme activity in heterogeneous systems with insoluble substrate or immobilized enzyme.
    Anal. Biochem. 221, 213-214.
  • E.Piotraschke, A.Nurk, B.Galunsky, V.Kasche (1994)
    Genetic construction of catalytically active cross-species heterodimer penicillin G amidase.
    Biotechnol. Lett. 16, 119-124.
  • M.Dineva, B.Galunsky, V.Kasche, D.D.Petkov (1993)
    Phenylacetyl group as enzyme-cleavable aminoprotection of purine nucleosides.
    Bioorg. & Med. Chem. Lett. 3, 2781-2784.
  • N.Stambolieva, Z.Mincheva, B.Galunsky, V.Kalcheva (1992)
    Penicillin amidase-catalysed transfer of low specific acyl moiety: synthesis of 7-benzoxazolonyl-acetamido desacetoxycephalosporanic acid.
    Enzyme Microb. Technol. 14, 496-500.
  • A.Nadim, I.B.Stoineva, B.Galunsky, V.Kasche, D.D.Petkov (1992)
    Mass transfer induced interchange of the kinetic and thermodynamic control of enzymic peptide synthesis in biphasic water-organic systems.
    Biotechnol. Techniques 6, 539-544.
  • I.B.Stoineva, B.Galunsky, V.S.Lozanov, I.P.Ivanov, D.D.Petkov (1992)
    Enzymic synthesis design and enzymic synthesis of aspartame.
    Tetrahedron 48, 1115-1122.
  • V.Kasche, G.Michaelis, B.Galunsky (1991)
    Binding of organic solvent molecules influences the P1-P2 stereo- and sequence-specificity of a-chymotrypsin in kinetically controlled peptide synthesis.
    Biotechnol. Lett. 13, 75-80 (1991).
  • I.B.Stoineva, B.Galunsky, K.I.Voivodov, N.A.Stambolieva, D.D.Petkov (1989)
    Enzymic synthesis of aspartame by thermolysin and penicillin amidase.
    Proc. Fifth Int. Conf. on Chemistry and Biotechnology of Biol. Active Natural Products, Varna, Vol.1, pp. 289-301.
  • V.Kasche, U.Haufler, D.Markovsky, S.Melnik, A.Ziech, B.Galunsky (1987)
    Penicillin amidase from E.coli - enzyme heterogeneity and stability.
    Anals New York Acad. Sci. 501, 97-102.
  • V.Kasche, B.Galunsky, D.Petkov (1987)
    Biotechnologische grundlagen der enzymkatalysierten semisynthese von kondensationsprodukten.
    Comm. Dept. Chem. Bulg. Acad. Sci. 20, 192-200.
  • V.Kasche, B.Galunsky, U.Haufler, R.Zöllner (1983)
    Kinetically vs. equilibrium-controlled synthesis of C-N bonds in -lactams and peptides with free and immobilized biocatalysts.
    In: Enzyme Technology (R.M. Lafferty Ed.), Springer Berlin, pp. 297-308.
  • V.Kasche and B.Galunsky (1982)
    Ionic strength and pH effects in the kinetically controlled synthesis of benzylpenicillin by nucleophilic deacylation of free and immobilized phenylacetyl-penicillin amidase with 6-amino-penicillanic acid.
    Biochem. Biophys. Res. Comm. 104, 1215-1222.
  • V.Kasche and B.Galunsky(1982)
    Ligand/ligate interactions in the heterogeneous systems: their influence on operational properties of affinity chromatographic and binding assay adsorbrnts.
    In: Affinity Chromatography and related Techiques, Elsevier Amsterdam, pp. 93-110.
  • Woiwodow, K., Galunsky, B., Dyankov, S., Gorinova,N., Tzakov, D. (1982)
    Immobilizierte Säure Protease zur Eiweißstabilisierung von Weinen.
    Mitt. Klosterneuburg 32, 117-123.
  • V.Kasche, B.Galunsky (1982)
    Kinetically controlled synthesis via acyl-enzyme intermediates using free and immobilized hydrolases.
    In: Biotechnology of Biologically Active Natural Products (R.Vlahov, ed.) Bulgarian Academy of Science, Sofia.
  • V.Kasche, K.Buchholz, B.Galunsky (1981)
    Resolution in high-performance liquid affinity chromatography. Dependence on eluite diffusion into the stationary phase.
    J. Chromatogr. 216, 169-174.
  • K.I.Voivodov, B.Galunsky, S.S.Dyankov (1979)
    Influence of silanization conditions on the activity of pepsin immobilized on silica gel.
    J. Appl. Biochem. 1, 442-447.
  • B.Galunsky, V.I.Kanazirev, K.I.Voivodov, S.S.Dyankov (1978)
    A new method for determining amino and isithiocyanate groups on the surface of inorganic supports for immobilized enzymes.
    Compt. rend. Acad. bulg. Sci. 31, 999-1002.
  • B.Galunsky, K.I.Voivodov, S.S.Dyankov (1978)
    Immobilization of alkaline mesentericopeptidase on NCS-modified silochrom.
    Compt. rend. Acad. bulg. Sci. 31, 1601-1604.
  • M.Kirilov, J.Petrova, S.Momchilova and B.Galunsky (1976)
    Reactionsfähigkeit von Estern der 2-Thienylmethanphosphonsäuere und deren Carbanionen.
    Chem. Ber. 109, 1684-1693.
  • M.Kirilov, J.Petrova, B.Galunsky, D.Nikolova (1976)
    Reactions of esters of 2-furyl and 2-pyridylemethanephosphonic acid with schiff bases in the presence of sodium amide.
    Compt. rend. Acad. bulg. Sci. 29, 379-382.


  • Kasche, V., Ignatova, Z., Galunsky, B. (2004)
    Gentechnisch veränderte Penicillinamidase und Verfahren zu ihrer Herstellung.
    DE 102 51 747 A1, 19.05.2004.

  • I.B.Stoineva, B.P.Galunsky, K.I.Voivodov, N.A.Stambolieva, D.D.Petkov, L.A.Lyublinskaya, M.A.Yusupova, E.K.Kotlova, V.M.Stepanov, G.G.Chestuhina (1991)
    p-Substituted phenylacetyl derivatives of aspartame and method for their synthesis.
    Bulg. Patent 48514.

  • I.B.Stoineva, B.P.Galunsky, K.I.Voivodov, N.A.Stambolieva, D.D.Petkov (1991)
    Enzymatic method for synthesis of aspartame.
    Bulg. Patent 48282.

  • K.I.Voivodov, N.A.Stambolieva, I.B.Stoineva, B.P.Galunsky, D.D.Petkov (1991)
    Heterogeneous biocatalysts for peptide synthesis.
    Bulg. Patent 48281.

  • N.A.Stambolieva, K.I.Voivodov, I.B.Stoineva, B.P.Galunsky, D.D.Petkov, I.B.Rashkov (1991)
    Heterogeneous biocatalyst for peptide synthesis.
    Bulg. Patent 48280.

  • D.D.Petkov, B.P.Galunsky, K.I.Voivodov, Y.N.Stefanovski (1979)
    Method for synthesis of D(-)&alpha-phenylglycine chloride hydrochloride.
    Bulg. Patent 26739.

  • L.V.Kozlov, Y.I.Krilova, B.P.Galunsky, V.K.Antonov(1979)
    Method for immobilization of acid proteinases.
    USSR Patent 693990.


New PhD position at ITB. Topic: Experimental and theoretical processing of biocatalytic cascade reactions.

New PhD position at ITB.

Topic: Experimental and theoretical processing of biocatalytic cascade reactions.

Complete offer information available here.

Edgar Gamero joins the ITB for his Master Thesis. Welcome!

Edgar Gamero joins the ITB for his Master Thesis. Welcome!

Edgar will work in the topic "Etablierung einer Hochzelldichtefermentation und eines Aktivitätstest zur Oxidation von Alkanen".

Good luck and welcome!

Dr. Martina Schedler has succesfully completed her PhD!

Martina Schedler has succesfully completed her PhD "Microbial degradation of crude oil at high pressure".


Aparna Nair joins the ITB for her Master's Project Work. Welcome!
Aparna Nair joins the ITB for her Master's Project Work. Welcome!

Aparna will work in the topic "Optimization of crude oil fractionation procedures for biodegradation analysis at high pressure conditions".

Good luck and welcome!