TUHH> ITB

Institute of Technical Biocatalysis

Publications of Prof. em. fil. Dr. Kasche (retired 2004)


Type Year
Publications in journals 2005, 2004 , 2003, 2002, 2001, 2000, -1999
Books & book chapter -2005
other publications -2005
patents -2005


Publications in journals

2005

  • S. C. Viegas, D. Schmidt, V. Kasche, C. M. Arraiano, Z. Ignatova
    Effect of the increased stability of the penicillin amidase mRNA on the protein expression levels
    FEBS Lett., 579, 5069-5073 (2005)
  • Z. Ignatova, F. Wischnewski, H. Notbohm, V. Kasche
    Pro-sequence and Ca2+-binding: Implications for folding and maturatiopn of Ntn-hydrolase Penicillin amidase from E. coli
    J.Mol. Biol. 348, 999-1014 (2005)
  • V. Kasche, Z. Ignatova, H. Märkl, W. Plate, N. Punckt, D. Schmidt, K. Wiegandt, B. Ernst
    Ca2+ is a cofactor required for membrane transport and maturation and is a yield determining factor in high cell density penicillin amidase production.
    Biotechnol. Prog. 21, 432-438 (2005)

2004

D. BOZHINOVA, B. GALUNSKY, G. YUEPING, M. FRANZREB, R. KÖSTER, V. KASCHE
Evaluation of magnetic polymer micro-beads as carriers of immobilised biocatalysts for selective and stereoselective transformations
Biotechnology Letters, 26, 343-350 (2004)

2003

  • V. Kasche, Boris Galunsky, Z. Ignatova
    Fragments of Pro-peptide Activate Mature Penicillin Amidase of Alcaligenes faecalis
    Eur.J.Biochem. 270, 4721-4728 (2003)
  • V. KASCHE, M. DE BOER, C. LAZO, M. GAD
    Direct observation of intraparticle equilibration and the rate limiting step in adsorption of proteins in chromatographic adsorbents with confocal laser scanning microscopy (CLSM)
    J. Chromatog. B 790, 115-130 (2003)
  • Z. Ignatova, A. Mahsunah, M. Georgieva, V. Kasche
    Improvement of Posttranslational Bottlenecks in the Production of Penicillin Amidase in Recombinant Escherichia coli strains
    Appl.Environ.Microbiol. 69, 1237-1245,(2003)

2002

  • B. GALUNSKY, V. KASCHE
    Determination of the Enantioselectivity for Kinetically Controlled Condensations Catalysed by Amidases and Peptidases
    ADV.SYNTH.CATAL. 344, 1115-1119(2002)
  • Z. IGNATOVA, C. HÖRNLE, A. NURK, V. KASCHE
    Unusual Signal Peptide Ddirects Penicillin Amidase from Escherichia coli to the Tat Translocation Machinery
    Biochem.Biophys.Res.Commun. 291, 146-149 (2002)

2001

  • V. KASCHE
    Physicochemical boundaries in the continuous and one-step discontinuous affinity-chromatographic isolation of proteins
    J. biochem biophys. methods, 49, 49-62 (2001)
  • A. SPIESS, V. KASCHE
    Enzymatic synthesis of amoxicillin. Process integration using multiphase systems
    Novel Frontiers in the Production of Compounds for Biomedical Use (A. van Broekhoven et al. eds) Kluwer Academic Publishers, Amsterdam, 169-192 (2001)
  • A.C. CHICERE, B. GALUNSKY, V. SCHÜNEMANN AND V. KASCHE
    Stability of immobilised soybean lipoxygenases: infulence of coupling conditions on the ionisation state of the active site Fe
    Enzyme Microb. Technol, 28, 168-175 (2001)
  • V. KASCHE
    Proteases in Peptide synthesis
    Proteases a practical approach (I. Bond and R. Beynon, eds.) 2nd edition, Oxford Univ. Press, Oxford, 265-292 (2001)
  • A. SPIESS, V. KASCHE
    Direct measurement of pH profiles in immobilised enzymes during kinetically controlled synthesis using CLSM
    Biotechnol Progress., 17, 294-303 (2001)
  • B. MAHRO, R. MÜLLER, V. KASCHE
    Bioavailability � the Key Factor of Soil Bioremediation
    Springer Verlag Berlin Heidelberg, 181-195, (2001)
  • V. KASCHE, A. SPIESS
    Enzymatic Transformations in suspensions
    Enzymes in Nonaqueous Media (P. Halling, B. Holland, E. Vulfson and J. Woodley (eds).) (2001)

2000

  • A.J.J. Straathof, V. Kasche
    Process conceptions for bioconversions
    Applied Biocatalysis, harwood academic publishers, in press, (2000)
  • L. HEWITT, V. KASCHE, K. LUMMER, R.J. LEWIS, G.N. MURSHUDOV, G.G. DODSON AND K.W. WILSON
    Structure of a slow processing precursor penicillin acylase from Escherichia coli reveals the linker peptide blocking the active site cleft
    J. Mol. Biol., 302, 887-898 (2000)
  • Z. IGNATOVA, S. TARUTTIS, V. KASCHE
    Role of the intracellular proteolysis in the production of the periplasmtic penicillin amidase in Escherichia coli
    Biotechnol. Letters, 22, 1727-1732 (2000)
  • A.C. CHIKERE, B. GALUNSKY, A. OVERMEYER, G. BRUNNER, V. KASCHE
    Activity and stability of immobilised soybean lipoxygenase-1 in aqueous and supercritical carbon dioxide media
    Biotechnol. Letters 22, 1815-1821 (2000)
  • BORIS GALUNSKY, KARSTEN LUMMER, VOLKER KASCHE
    Comparative Study of Substrate- and Stereospecificty of Penicillin G Amidases from Different Sources and Hybrid Isoenzymes
    Chem. MONTHLY, 131, 623-632 (2000)
  • Z. IGNATOVA, S.-O. ENFORS, S. HOBBIE, S. TARUTTIS, C. VOGT, V. KASCHE
    The relative importance of intracellular proteolysis and transport on the yield of the periplasmatic enzyme penicillin amidase in ESCHERICHIA COLI
    Enzyme Microb. Technol., 26 165-170 (2000)

 

-1999

  • V. KASCHE, K. LUMMER, A. NURK, E. PIOTRASCHKE, A. RIEKS, S. STOEVA, W. VOELTER
    Intramolecular autoproteolysis initiates the maturation of penicillin amidase from E. coli
    Biochem. Biophys. Acta, 1433, 76-86 (1999)
  • K. LUMMER, A. RIECKS, B. GALUNSKY, V. KASCHE
    pH-dependence of penicillin amidase enantioselectivity for charged substrates
    Biochem. Biophys. Acta, 1433, 327-334 (1999)
  • L. HEWITT, V. KASCHE, K. LUMMER, A. RIEKS, K. WILSON
    Crystallization of a precursor penicillin acylase from Escherichia coli
    Acta. Crystallog., 55, 1052-1054, (1999)
  • W. TISCHER, V. KASCHE
    Immobilized Enzymes: Crystals or Carriers?
    TIBTECH, 17, 326-335, (1999)
  • A. OVERMEIER, S. SCHRADER-LIPPELT, V. KASCHE, G. BRUNNER
    Lipase-catalysed kinetic resolution of racemates at temperatures from 400C to 1600 in supercritical carbon dioxide
    Biotechnol. Letters, 21, 65-69 (1999)
  • K. LÜDTKE, K.-V. PEINEMANN, V. KASCHE, R.-D. BEHLING
    Nitrate removal of drinking water by means of catalytically active membranes
    J. Membrane Science, 151, 3-12, (1999)
  • A.SPIESS, R.SCHLOTHAUER, J.HINRICHS, B.SCHEIDAT, V. KASCHE
    pH gradients in heterogeneous biocatalysts and their influence on rates and yields of the catalysed processes
    Biotechnol. Bioeng., 62, 267-277 (1999)
  • ANTHONY CHIKERE, BORIS GALUNSKY, VOLKER KASCHE
    Soybean Lipopxygenases: Purification and Stability of the Free and Immobilized Enzymes
    Progress in Biotechnology (A. Ballesteros, F.J. Plou, J.L. Iborra., P.J. Halling (eds) Elsevier Science, Amsterdam, 15, 559-564 (1998)
  • Z. IGNATOVA, S. STOEVA, B. GALUNSKY, C. HÖRNLE, A. NURK, E. PIOTRASCHKE, W. VOLTER, V. KASCHE
    Proteolytic processing of penicillin amidase from Alcaligenes faecalis cloned in E. coli yields several active forms
    Biotechnology Letters, 20, 977-982 (1998)
  • B. GALUNSKY, S. IGNATOVA. V. KASCHE
    Temperature effects on S1- and S1`-enantionselectivity of alpha-Chymotrypsin
    Biochem. Biophys. Acta. 1343, 130-138 (1997)
  • N. GOTTSCHLICH, V. KASCHE
    Purification of monoclonal antibodies by simulated moving-bed chromatography
    J. Chromatog.765, 201-206 (1997)
  • I. Ivanov, V. KASCHE, D. PETKOV
    Thermodynamics of Enzymic Peptide Synthesis in Biphasic Aqueous-Organic Systems: Coupling of the Chemical and Phase Equilibria
    Biocatalysis and Biotransformation 14, 181-194 (1997)
  • B. MAHRO, A. ESCHENBACH, L. LEHNE, G. SCHAEFER, R. WIENBERG, V. KASCHE
    Möglichkeiten und Grenzen zur Beeinflussung des biologischen PAK-Abbaus
    Neue Techniken der Bodenreinigung (Re. Stegmann) Economica Verlag, p. 317-329 (1996)
  • O. GUTHMANN, R. SCHWERDTFEGER, A. RIEKS, G. ANTRANIKIAN, V. KASCHE, G. BRUNNER
    Enzyme catalysed reactions, enantioselectivity and stability under high hydrostatic pressure
    High Pressure Chemical Engineering (Ph. R. von Rohr und Ch. Trepp, Hrsg.) Elsevier Science, 127-131 (1996)
  • V. KASCHE, B. GALUNSKY, A. NURK, E. PIOTRASCHKE, A. RIEKS
    The dependeny of the stereoselectivity of penicillin amidases - enzymes with R-specific S1- and S-specific S�1-subsites- on temperature and pimary structure
    Biotechnol. Letters, 18, 455-460 (1996)
  • N. GOTTSCHLICH, S. WEIDGEN, V. KASCHE
    Continuous biospecific affinity purification of enzymes by simulated moving bed chromatography: Theoretical description and experimental results
    J. Chromatog. 719, 267-274 (1996)
  • V: KASCHE
    An integrated approach in the analytical description of affinity chromatography, biosensors, immobilized biocatalysts and similar systems
    Adv. in Mol. Cell. Biol. 15.B 605-620 (1996)
  • B. MAHRO, K. ROHDE, V. KASCHE
    Non-Selective Precultivation of Bacteria Able to Degrade Different Polycyclic Aromatic Hydrocarbons (PAH)
    Acta Biotechnol. 15, 337-345 (1995)
  • M. KLINGEBERG, B. GALUNSKY, C. SJÖHOLM, V. KASCHE, G. ANTRANIKIAN
    Purification and properties of highly thermostable, SDS resistant and stereospecific proteinase from the extreme thermophilic archeon Thermococcus stetteri
    Appl. environm. Microbiol., 61, 3098-3104 (1995)
  • V. KASCHE, B. GALUNSKY
    Enzyme catalyzed biotransformations in aqueous two-phase systems with precipitated substrate and/or product
    Biotechnol. Bioeng. 45, 261-267 (1995)
  • V. Kasche, G. Schietke, J. Behnert, H.-J. Thes Probenaufarbeitungssystem für Bio- und Umwelttechnik
    BIOforum, 17, 138-140 (1994)
  • B. GALUNSKY, R. SCHLOTHAUER, B.BÖCKLE, V.KASCHE
    Direct spectrometric measurement of enzyme activity in heterogeneous systems with insoluble substrate or immobilized enzyme
    Anal. Biochem. 221, 213-214 (1994)
  • E. PIOTRASCHKE, A. NURK, B. GALUNSKY, V. KASCHE
    Genetic construction of catalytically active cross-species heterodimer penicillin G amidase
    Biotechnol. Letters 16, 119-124 (1994)
  • O.C. AWORH, V. KASCHE,. and O.O. APAMPA
    Purification and some properties of Sodom Apple Latex Proteinases
    Food Chem. 50, 359-362 (1994)
  • V. KASCHE, N. GOTTSCHLICH, A. LINDBERG, C. NIEBUHR- REDDER, AND J. SCHMIEDING
    Perfusible and non-perfusible supports with monoclonal antibodies for biospecic purification of E.coli penicillin amidase within its pH-stability range
    J.Chromatog.660, 137-145 (1994)
  • A. BALLESTEROS, K. BUCHHOLZ, L. BOROSS, J. CABRAL and V. KASCHE
    Biocatalyst performance
    Applied Biocatalysis (J. Cabral, D. Best, L. Boross, J. Tramper, eds.), Chapt. 5, Harwood academic publishers, Chur, Kap. 5 (1994)
  • A. KÄÄRD, A. SCHWELLENBACH, V. KASCHE, E. RENKEN
    A fluorescence based penicillin biosensor for on-line monitoring of benzylpenicillin hydrolysis. Optimization of the fluorescence signal for the measurement of benzylpenicillin
    J.Anal.Chim. (Russ.Akad.Wiss.) 48, 306-312 (1993)
  • M.A. DINEVA, B. GALUNSKI, V. KASCHE, and D. PETKOV
    Phenylacetyl group as enzyme-cleavable aminoprotection of purine nucleotides
    Bioorg.Medic.Chem Letters, 3, 2781-2784 (1993)
  • V. KASCHE, W. HAß, U. HAUFLER, G. MICHAELIS, C. NIEBUHR-REDDER, Th. SCHOLZEN, and T. WIESEMANN
    Penicillin Amidase from E.coli
    50 years of penicillin application (H. Kleinkauf, and H.v. Döhren, eds.) Public Ltd, Prague, 291-303 (1993)
  • E.K. BRATOVANOVA, V. KASCHE, D.D. PETKOV
    Kinetically-controlled enzymic synthesis of ribonucleotide bond in vitro
    Biotechnol. Letters 15, 347-352 (1993)
  • O.C. AWORH and V. KASCHE
    Purification an preliminary characterization of sodom apple proteinase
    DECHEMA Biotechnology Conferences 5, 137-140 (1992)
  • V.KASCHE, W. HAß, C. NIEBUHR-REDDER, and E. PIOTRASCHKE
    Penicillin Amidase aus E.coli - ein biotechnisches Enzym auf der Suche nach einer biologischen Funktion
    BioEngineering 8, 69-77 (1992)
  • A. BOHMANN, R. PÖRTNER, J. SCHMIEDING, V. KASCHE AND H. MÄRKL
    The membrane dialysis bioreactor with integrate radial-flow fixed bed - a new approach for continuous cultivation of animal cells
    Cytotechnology 9, 51-57 (1992)
  • B. MAHRO, A. PETERMANN-HERMANN, S. WALENTA, W. MÜLLER-KLIESER, and V. KASCHE
    Non-extractive localization and imaging of luminescent bacteria in liquid and soil samples by luminescence microscopy
    Microb. Releases 1, 79-85 (1992)
  • A.NADIM, I.B.STOINEVA, B.GALUNSKY, V. KASCHE and D. PETKOV
    Mass transfer induced interchange of the kinetic and thermodynamic control of enzymic peptide synthesis in biphasic water-organic systems
    Biotechnology Techniques 6, 539-544 (1992)
  • M. SCHUSTER, V. KASCHE, H.-D. JAKUBKE
    Contribution to the S�-subsite specificity of papain
    Biochem.Biophys.Acta, 1121, 207-212 (1992)
  • V. KASCHE, E.RENKEN, G, SCHIETKE, R. ULRICH, R. BÜCKE, H. GNEWUCH, K. JANSEN
    Signal generation and evaluation in fluorescence based on-line fiber optic biosensors
    Biosensors: Fundamentals, Technologies and Applications (F. Scheller, R.D. Schmid, eds.) VCH Verlagsgesellschaft mbH, Weinheim p. 265-274 (1992)
  • LESLEY J. BARTUNIK, HANS D. BARTUNIK, LOTHAR R. JACOB, VOLKER KASCHE AND THOMAS WIESEMANN
    Crystallization and preliminary x-ray data of proteolitically processes penicillin amidase PA 6.7 from Escherichia Coli
    (1991)
  • M. Schuster, H.-D. Jakubke and V. Kasche
    Nucleophile specificity in papain-catalyzed acyl transfer reactions
    Biomed.Biochim.Acta 50, 12-126 (1991)
  • C. NIEBUHR-REDDER, V. KASCHE
    Antibodies to E. coli Penicillin G Amidase for biochemical studies and the control of fermentation processes
    Proceedings of the German-Japanese Workshop on animal cell culture technology, December 4-5 1990, Japan Society for the Promotion of Science, 57-60 (1991)
  • V. KASCHE, G. MICHAELIS, T. WIESEMANN
    Stereo-and sequence specificity of serine proteases in peptide synthesis
    Biomed. Biophys. Acta 50, 38-43 (1991)
  • V. KASCHE, B. GALUNSKY, and G. MICHAELIS
    Binding of organic solvent molecules influences the P1'-P2' stereo and sequence specificity of a-chymotrypsin in kinetically controlled peptide synthesis
    Biotechnology Letters 13, 75-80 (1991)
  • B. MAHRO, L. SCHLEINKOFER, V. KASCHE
    In situ detection and localization of bioluminescent Streptomyces coelicolore in liquid and soil by photon imaging
    Acta. Biotechnol. 11, 541-546 (1991).
  • V: SCHELLENBERGER, H.-D. JAKUBKE, and V.KASCHE
    Electrostatic effects in the alfa-chymotrypsin-catalyzed acyl transfer II. Efficiency of nucleophiles bearing charged groups in various locations
    Biochem.Biophys.Acta 1078, 8-11 (1991).
  • A. Käärd, V. Kasche, E. Renken and G. Schietke
    FLUORESCENCE BASED PH BIOSENSOR FOR ON-LINE MONITORING OF BENZYLPENICILLIN-HYDROYLSIS (IN RUSSIAN)
  • E. RENKEN, A. KÄÄRD, and V. KASCHE
    Fluorescence of proteins adsorbed to chromatographic supports: Application in studies on adsorption - desorption kinetics
    Fundamentals of Adsorption (A.B. Mersmann, S.E. Schol, eds.) United Eng. Trustees Inc, New York, p. 715-722 (1991)
  • M. KÄSTNER, B. MAHRO, V. KASCHE
    Biologische Abbaubarkeit von Schadstoffen
    Reinigung kontaminierter Böden (R. Stegmann, V. Franzius eds.) Economica Verlag, p. 27-47 (1990)
  • G.MICHAELIS, and V. KASCHE
    S1'- subsite mapping of penicillin amidase in the kinetically controlled peptide synthesis
    DECHEMA Biotechnology Conferences, 4, 293 (1990
  • E. RENKEN, A. KÄÄRD, G. SCHIETKE, and V. KASCHE
    Automatic online monitoring of benzylpenicillin hydrolysis with a fluorescnec-based penicillin biosensor
    DECHEMA Biotechnology Conferences, 4, 949 (1990)
  • V. KASCHE, and R. ULRICH
    Fluorescence Biosensors: Signal generation and signal transmission with fiber optics
    Fresenius J. Anal. Chem. 337, 24-25 (1990)
  • V. KASCHE, T. SCHOLZEN, TH. BOLLER, D.M. KRÄMER, F. LÖFFLER
    Rapid Protein Purification using PBA-EUPERGITTM a novel method for Large-Scale Procedures
    J. Chromatog. 510, 149-154 (1990)
  • E. RENKEN, V. KASCHE, A. KÄÄRD
    Optimization of the Signals of Biosensors based on pH dependent Fluorescence Changes
    DECHEMA Biotechnology Conferences Vol. 3, 743-746 (1990)
  • B. MAHRO, V. KASCHE, L. SCHLEINKOFER
    Photon Imaging: A powerful tool for the environmental monitoring of genetically engineered micoroorganisms
    DECHEMA Biotechnology Conferences Vol. 3, Frankfurt, 447-452 (1990)
  • V. KASCHE, G. MICHAELIS
    Protease catalysed peptide synthesis in systems with organic solvents
    DECHEMA Biotechnology Conferences Vol. 3, 237-240 (1990)
  • V. KASCHE und G. MICHAELIS
    Peptide Bond Synthesis by Enzyme-Catalyzed Acyl-Transfer
    in: Biochemistry of Peptide Antibiotics, (Kleinkauf and v.Döhren, eds.), W. de Gruyter, Berlin, 81-101 (1990) 81. VOLKER KASCHE, GUNTER SCHIETKE, JÜRGEN BEHNERT UND KURT-PETER RAEZKE Prozessüberwachung und-steuerung von Bioreaktoren. In BioTec, Juli 1990
  • E. Renken, A. Käärd, and V. Kasche
    Fluorescence of Proteins Adsorbed to chromatographic supports: Application in studies on adsorption-desorption kinetics
    Fundamentals of Adsorption. Eng.Formel.Conf. May 1989
  • V. KASCHE and E. RENKEN
    Biosensoren - Sensoren fÜr die Biotechnik
    Chem.-Ing.-Tech. 61,.783-787, (1989)
  • V. KASCHE, A. KÄÄRD, E. RENKEN, H. GREWE, K. JANSEN and R. ULRICH
    DYNAMIC fluorescence biosensors: Principles of signal generation and of coupling to optical-fibers
    Biosensors: application in medicine, environmental protection, and process control, (R. D. Schmid and F. Scheller. eds.), Weinheim, Basel, Cambridge, New York,) p. 233-242 (1989
  • J. DITTMER, A. DITTMER, R. DELLA BRUNA, V. KASCHE
    A native, affinity-based protein blot for the analysis of streptavidin heterogeneity: Consequences for the specificity of streptavidin mediated bindung assays
    Electrophoresis, 10, 762-765 (1989)
  • V. KASCHE
    Proteases in peptide synthesis
    Proteases a practical approach( R. Beynon, and J. Bond eds.) IRL Press, Oxford, 125-143 (1989)
  • A. SCHÖNE, M. ZIERUL, V. KASCHE
    Simulation and model-based prozess control of continuously operated fermentation processes for the production of microbial metabolites
    DECHEMA-BIOTECHNOLOGY-CONFERENCES vol. 2, VCH, Weinheim Frankfurt, in press, 117-130 (1988)
  • V. KASCHE, R. SCHLOTHAUER and G. BRUNNER
    Enzyme denaturation in supercritical CO2: Stabilizing effect of S-S bonds during the depressurization step
    Biotechnol. Letters, 10, 569-574 (1988)
  • U. HAUFLER, R. ULMKE, T. WIESEMANN, and V. Kasche
    Structure, pH-stability and renaturation of free and immobilized E. coli penicillin Amidase
    Biotechnology in Germany (H.J. Rehm, ed.) Verlag Chemie, Weinheim, 345-350 (1988)
  • V. KASCHE
    Hydrolases as biocatalysts for the synthesis of biologically active condensation products (b-lactam antibiotics and peptides)
    Biotechnology of biologically active natural products (R. Vlakov, ed.) Bulgarian Academy of Science
  • V. KASCHE, B. GALUNSKY, and D. D. PETKOV
    Biotechnologische Grundlagen der enzymkatalysierten Synthese von Kondensationsprodukten
    Isvestija po chimia, 20, 192 (1987)
  • V. KASCHE
    Mechanisms and maximum yield in kinetically controlled peptide synthesis
    Advances in the Biosciences, 65, 151-158 (1987).
  • V. KASCHE, U. HAUFLER, and L. RIECHMANN
    Equilibrium and Kinetically Controlled Synthesis with Enzymes: Semisynthesis of Penicillins and Peptides
    Methods in Enzymology, 136, 280-292 (1987)
  • V. KASCHE, U. HAUFLER, D. MARKOWSKY, S. MELNYK, and A. ZEICH
    Penicillin Amidase from E. coli-enzyme heterogeneity and stability
    Ann. N.Y. Acad. Sci., 501, 97-102 (1987)
  • V. KASCHE
    Mechanism and yields in enzyme catalyzed equilibrium and kinetically controlled synthesis of �-lactam antibiotics peptides and other condensation products
    Enzyme Microb. Technol., 8, 4-16 (1986)
  • L. RIECHMANN, and V. KASCHE
    Reaction mechanism, specificity and pH dependence of peptide synthesis catalyzed by the metalloproteinase thermolysin
    Biochim. Biophys. Acta, 872, 269-276 (1986)
  • J. BULLERDIEK, J. DITTMER, A. FAEHRE, S. BARTNITZKE, V. KASCHE, and W. SCHLOOT
    A new banding pattern of human chromosomes after nick-translation using eco RI and biotin-dUTP
    Clin. Genet., 28, 173-178 (1985)
  • V. KASCHE
    Ampicillin and cephalexin synthesis catalyzed by E. coli penicillin amidase yield increase due to substrate recycling
    Biotechnol. Letters 7, 877-882 (1985)
  • L. RIECHMANN, and V. KASCHE
    Peptide synthesis catalyzed by the serine proteases chymotrypsin and trypsin
    Biochem. Biophys. Acta., 830, 164-172 (1985)
  • V. KASCHE, and U. HAUFLER
    Enzyme catalyzed semisynthesis of condensation products - kinetic vs. equilibrium control
    Third European Congress of Biotechnology, Verlag Chemie, Weinheim, 425-434 (1984)
  • V. KASCHE, U. HAUFLER, and R. Z�LLNER
    Kinetic studies on the mechanism of the penicillin amidase catalyzed synthesis of ampicillin and benzylpenicillin
    Hoppe Seyler's Z. Physiol. Chem., 365, 1435-1443 (1984)
  • V. KASCHE, and U. HAUFLER
    Ausbeutebestimmende Faktoren bei der enzymkatalysierten Synthese von semisynthetischen beta-Lactamamantibiotica
    DECHEMA Monographs, Vol. 95, Verlag Chemie, Weinheim, S. 33-49 (1984)
  • V. KASCHE, U. HAUFLER, l. RIECHMANN, und R. ZÖllner
    Kinetically controlled semisynthesis of beta-lactam antibiotics and peptides
    Ann. N.Y. Acad. Sci., 434, 99-105 (1984)
  • L. RIECHMANN, and V. KASCHE
    Kinetic studies on the mechanism and specificity of peptide semisynthesis catalyzed by the serine proteases alpha-chymotrypsin and beta-trypsin
    Biochem. Biophys. Res. Comm., 120, 686-691 (1984)
  • L. RIECHMANN, and V. KASCHE
    Sequence specificity in proteinase-catalyzed peptide semi-synthesis - does it reflect substrate specificity
    Hoppe Seyler's Z. Physiol. Chem., 364, 1200 (1983)
  • V. KASCHE, B. GALUNSKY, U. HAUFLER, and R. ZÖLLNER
    Kinetically vs. equlibrium-controlled synthesis of C-N bonds in beta-lactams and peptides with free and imobilized biocatalysts
    Enzyme Technology (R.M. Lafferty, ed.), Springer Verlag, 297-308 (1983)
  • V. KASCHE
    Correlation of theoretical and experimental data for immobilized biocatalysts
    Enzyme Microbiol. Technol., 5, 2-13 (1983)
  • R. AGUIRRE, and V. KASCHE
    Catalytically active monomer forms of immobilized arginase
    Eur.J.Biochem., 130, 373-381 (1983)
  • P. ZIPFEL, J. DITTMER, V. KASCHE, and H. AMNEUS
    DNA-turnover in cultured animal cells
    Hoppe Seyler's Z. Physiol. Chem., 363, 937 (1982)
  • V. KASCHE, and R. ZÖLLNER
    Tris (hydroxylmethyl) methylamine is acylated when it reacts with acyl-chymotrypsin
    Hoppe Seyler's Z. Physiol. Chem., 363, 531-534 (1982)
  • K. BUCHHOLZ, A. BORCHERT and V. KASCHE
    Aspects of affinity chromatography in HPLC
    Practical Aspects of Modern High Performance Liquid Chromatography (J. Molnar, ed.) Walter de Gruyter, Berlin, New York, 187-196 (1982)
  • V. KASCHE, and B. GALUNSKY
    Ionic strength and pH-effects in the kinetically controlled synthesis of bezylpenicillin by nuleophilic deacylation of free and immobilized phenyl-acetyl-penicillin amidase with 6-Aminopenicillanic adid
    Biophys. Biochem. Res. Comm., 104, 1215-1222 (1982)
  • V. KASCHE, and B. GALUNSKY
    Kinetically controlled synthesis via acyl-enzyme intermediates using free and immobilized hydrolases
    Biotechnology of Biologically Active Natural Products (R.Vlahov, ed.) Bulgarian Academy of Science, Sofia (1982)
  • V. KASCHE, and B. GALUNSKY
    Ligand/Ligate interactions in heterogeneous systems: Their influence on operational properties of affinity chromatographic and binding assay adsorbents
    Affinity Chromatography IV (T. Gribnau and J. Visser, eds.), Elsevier-North, Holland, Publ. Co, Amsterdam (1982)
  • V. KASCHE, B. GALUNSKY and K. BUCHHOLZ
    Resolution in high performance liquid affinity chromatography. Dependence on eluite diffusion into the stationary phase
    J. Chromatogr., 216, 169-174 (1981)
  • V. KASCHE, and I. BÜCHTMANN
    A method for the isolation of homogeneous fluoresceinla-belled proteins for studies on the variation in biological and fluorescence properties of the different konjugates
    Hoppe Seyler's Z. Physiol. Chem., 362, 1007-1012 (1981)
  • V. KASCHE, R. Z�LLNER, H. AMNEUS, and L. NÄSLUND
    A two-step procedure for quantitative isolation of pure double strand DNA from animal tissue and cell cultures
    Prep. Biochem. 11, 233-251 (1981)
  • V. KASCHE
    Stimulus-secretion coupling as an effector mediated self organisation process involving membrane fusion
    Self-organizing Systems (G. Roth and H. Schwegler, eds.) Campus Verlag, Frankfurt, 73-83 (1981)
  • V. KASCHE, K. PROBST, and J. MAASS
    The DNA, RNA and trypsin content in trypsins used in monolayer animal cell culture studies
    Cell Biol. Int. Reports, 5 , 1 (1981)
  • V. KASCHE, and I. BÜCHTMANN
    Purification of flurescein-labelled chymotrypsin by isoelectric focussing after biospecific adsorption to soybean trypsin inhibitor
    Structure and Activity of Natural Peptides (W. Voelter and G. Weitzel, eds.) Walter de Gryter Verlag, Berlin, 273-286 (1981)
  • V. KASCHE, and G. KUHLMANN
    Direct measurements of the thickness of the unstirred diffusion layer outside immobilized biocatalysts
    Enzyme Microbiol. Technol., 2 , 309-312 (1980)
  • V. KASCHE, and P. ZIPFEL
    Die spezifische AktivitÄt von Tritium und C-14 in DNA in Abhängigkeit von der spezifischen Aktivität der organischen und anorganischen Vorstufen in der Nahrung
    Strahlenschutzprobleme in Zusammenhang mit der Verwendung von Tritium und Kohlenstoff-14 und ihren Verbindungen (F. Stieve und G. Kistner, Hrsg.). Bundes�gesundheitsamt, STH Berichte 12/1980, 107-111 (1980)
  • V. KASCHE, and R. LANGENBERG
    The differences between plug-flow and continuous backmix reactors with immobilized biocatalysts. Can theoretical calculations predict the experimental behaviour?
    Food Process Engineering (P. Linko, Y. MÄlkki, J. Olkku and P. Larinkari, eds.) vol. 2, Applied Science Publ., Barking, England (1980)
  • V. KASCHE
    Characterization of biospecific adsorbents used for affinity chromatography
    Characterization of Immobilized Biocatalysts (K. Buchholz, ed.) DECHEMA Monographs, vol. 84, Verlag Chemie, Weinheim, 367-377 (1979)
  • V. KASCHE, and K. BUCHHOLZ
    Mass transfer influence on effectivness
    Characterization of Immobilized Biocatalysts (K. Buchholz, ed.) DECHEMA Monographs, vol. 84, Verlag Chemie, Weinheim, 208-243 (1979)
  • K. BUCHHOLZ, E. EHRENTHAL, M. GLOGER, N. HENNRICH, O. JAWOREK, V. KASCHE, J. KLEIN, D.M. KRÄMER, M.R. KULA, G. MANECKE, D. PALM, J. SCHLöNSEN, and F. WAGNER
    Characteristic properties and summary of determination methods
    Characterization of Immobilized Biocatalysts (K. Buchholz, ed.) DECHEMA Monographs, vol. 84, Verlag Chemie, Weinheim, 1-48 (1979)
  • I. BÜCHTMANN, and V. KASCHE
    Purification of fluorescein labelled chymotrypsin by isoelectric focussing after biospecific adsorption to soybean trypsin inhibitor
    Hoppe Seyler's Z. Physiol. Chem., 360 , 1136- ? (1979)
  • R. AGUIRRE, and V. KASCHE
    Affinity chromatography of subunit proteins: The reversible dissociation of sepharose-bound rat-liver arginase and its application in subunit exchange chromatography
    Hoppe Seyler's Z. Physiol. Chem., 360 , 222 (1979)
  • H. AMNEUS, D. GABEL, L. NÄSLUND, and V. KASCHE
    Biospecific sample reparation and application in isoelectric focussing
    Biochem. Biophys. Res. Comm., 90 , 1313-1320 (1979)
  • V. KASCHE, A. KAPUNE, and H. SCHWEGLER
    The operational effectiveness factor of immobilized enzyme systems
    Enzyme Microbiol. Technol., 1 , 41-46 (1979)
  • V. KASCHE
    Prozesse an Membranen - Einflu� der Mikroumgebung und Massentransports
    Zelluläre Kommunikations- und Kontrollmechanismen (L. Rensing und G. Roth, Hrsg.), Universität Bremen, Bremen, 13-26 (1978)
  • V. KASCHE, R. Z�LLNER, H. AMNEUS, and L. NÄSLUND
    A rapid and quantitative method to determine the tritium content in DNA from small tissue samples
    in: IAEA/NEA International Symposium on the Behaviour of Tritium in the Environment, Oct. 16-20 1978, International Atomic Energy Agency, Wien, 173-183 (1978)
  • V. KASCHE, A. KAPUNE, and H. SCHWEGLER
    Relative importance of diffusion layer resistance and microenvironmental effects on the effectivness of immo-bilized enzyme reactors
    Enzyme Engineering (G. Manecke, G. Brown, L.�Wingard, eds.), vol. 4, Plenum Press, New York, 365-371 (1978)
  • V. KASCHE, D. AMNEUS, and D. GABEL
    Physical-chemical factors influencing the resolution in affinity chromatography
    Enzyme Enginneering (G. Manecke, G. Brown, L. Wingard, eds.), vol. 4, Plenum Press, New York, 449-450 (1978)
  • A. KAPUNE, and V. KASCHE
    Kinetically controlled equilibria: The perturbation of hydrolysis equilibria in reactions catalyzed by alpha-chymotrypsin immobilized on charged supports
    Biochem. Biophys. Res., 80 , 955-962 (1978)
  • H. AMNEUS, V. KASCHE, D. GABEL, and H. LINDQVIST
    Affinity chromatography of chymotrypsin on soybean trypsin inhibitor sepharose: Applications in genetics and nucleide labelling
    Affinity Chromatography (Ostenhof et al., ed.), London: Butterworth, 99-102 (1977)
  • V. KASCHE, H. MANNWEILER, and P. ZIPFEL
    Dose estimations for tritium and 14C released in the nuclear fuel Cycle. A biological and radio-biological evaluation
    Quantifizierbarkeit des Strahlenrisikos (I. Schmitz-Feuerhake, Hrsg.) UniversitÄt Bremen, Bremen (1977)
  • V. KASCHE, H. AMNEUS, L. NÄSLUND, and R. Z�LLNER
    A rapid two-step procedure to isolate native DNA
    Hoppe Seyler's Z. Physiol. Chem., 358 , 256 (1977)
  • V. KASCHE, H. AMNEUS, and L. NÄSLUND
    Rapid zymogen activation and isolation of serine proteases from an individual mouse pancreas by affinity chroma-tography. Genetial heterogeneity of chymotrypsins of mus musculus
    Biochim. Biophys. Acta., 490 , l-18 (1977)
  • D. GABEL, and V. KASCHE
    Conformational transitions in immobilized enzymes
    Methods of Enzymology (K. Mosbach, ed.) vol. 44, Academic Press, New York, 526 (1976)
  • V. KASCHE, and A. KAPUNE
    Immobilized enzymes - their relevance as model systems for reactions at the cell surface: Influence of diffusion layer thickness on reaction rates
    Stud. biophys., 56 , 47-48 (1976)
  • D. GABEL, H. AMNEUS, and V. KASCHE
    Resolution in affinity chromatography
    J. Chromatogr., 120 , 391-397 (1976)
  • V. KASCHE
    The kinetics of trypsinogen activation studied by analytical affinity chromatography
    Arch. Biochem. Biophys., 173 , 269-272 (1976)
  • V. KASCHE
    Radiation-induced crosslinking of alpha-chymotrypsin to DNA and an agarose gel
    Int. J. Rad. Biol., 26 , 455-465 (1974)
  • V. KASCHE
    Oxygen effect: Differences in radiation-induced deactivation of mobile and immobilized enzymes
    Rad. Environm. Biophysics, 11 , 189-193 (1974)
  • V. KASCHE, and M. BERGWALL
    Intrinsic molecular properties and inhibition of immobilized enzymes. Theory and experimental observations on alpha-chymotrypsin-Sepharose
    Insolubilized Enzymes (M. Salmona, C. Saronio and S. Garattini, eds.) Raven Press, New York, 77-86 (1974)
  • V. KASCHE
    An affinity chromatographic study of trypsinogen activation. Kinetic evidence for the existence of neotrypsinogen
    Z. Klin. Chem. Klin. Biochem., 12 , 242 (1974)
  • D. GABEL, and V. KASCHE
    Autolysis of beta-trypsin. Influence of calcium ions and heat
    Acta Chem. Scand., 27 , 1971-1981 (1973)
  • V. KASCHE
    Effects of the microenviroment on the specific interacti-on between alpha-chymotrypsin and immobilized soybean trypsin inhibitor
    Studia Biophysica, 35 , 45-56 (1973)
  • D. GABEL, and V. KASCHE
    Cooperative transitions between active alpha-beta-trypsin conformations
    Biochem. Biophys. Res. Comm., 48 , 1011-1018 (1972)
  • V. KASCHE, H. Linqvist, R. Bergman, and R. AXEN
    A theoretical model describing steady state catalysis by enzymes immobilized in spherical gel particles. Experimental study of alpha-chymotrypsin-Sepharose
    Biochem. Biophys. Res. Comm., 45 , 615-621 (1971)
  • V. KASCHE
    Specific protein-protein interaction and its application in studies in radiation-induced protein modification
    I. Interaction of a-chymotrypsin with soybean trypsin inhibitor. II. Interaction of a-chymotrypsin with soybean trypsin inhibitor bound to a polysaccharide matrix. III. Radiation-induced modification of a-chymotrypsin. Acta Universitatis Upsaliensis, 2 , 1-132 (1971)
  • V. KASCHE
    Activity distributions in modified alpha-chymotrypsin
    Biochem. Biophys. Res. Comm., 38 , 875-881 (1970)
  • V. KASCHE
    Production and purification of 11C- and 13N-labelled alpha-chymotrypsin
    Radiochem. Radioanal. Letters, 3 , 51-56 (1970)
  • H. LINSCHITZ, D.R. CROSS, V.KASCHE, and J. TENENBAUM
    Low-temperature studies on phytochrome, light and dark reactions in the red to far-red transformation and intermediate forms of phytochrome
    Proc. U.S. Nat. Acad. Sci., 61 , 1095-11o1 (1968)
  • H. LINSCHITZ, and V. KASCHE
    Kinetics of phytochrome conversion. Multiple pathways in the PR to PFR reaction, as studied by double flash-technique
    Proc. U.S. Nat. Acad. Sci., 58 , 1059-1064 (1967)
  • V.KASCHE
    Radical intermediates in the fluorescein- and eosin- photosensitized autoxidation of 1-tyrosine
    Photochem. Photobiol., 6 , 643-650 (1967)
  • H. LINSCHITZ, V.KASCHE, W.L. BUTLER, and H.W. Siegelmann
    The kinetics of phytochrome conversion
    J. Biol. Chem., 241 , 3395-3403 (1966)
  • V.KASCHE, and L. LINDQVIST
    Transient species in the photochemistry of eosin
    Photochem. Photobiol., 4 , 923-933 (1965)
  • V. KASCHE, and L. LINDQVIST
    Reactions between the triplet state of fluorescein and oxygen
    J. Phys. Chem., 68 , 817-823 (1964)


Other publications

  • V. KASCHE
    Enzymtechnische Produktionsverfahren - können sie mit Gentechnik nachhaltiger gestaltet werden?
    Chancen und Risiken der Gentechnik im Umweltschutz. Thüringer Ministeriumn für Umweltschutz, Erfurt, 1998, S 166-174
  • V. KASCHE
    ENZYME ALS BIOKATALYSATOREN - NACHHALTIGE TECHNIK ZUR RESSOURCEN- UND UMWELTSCHONENDEN STOFFUMWANDLUNGEN FÜR DIE BEDARFSDECKUNG MIT MATERIELLEN GÜTERN ?
    Simone Leinkauf Hrsg. Metropolis Verlag Marburg, 1997
  • V. KASCHE
    Sensoren für die Biotechnik ?
    Chemische Industrie, April 1991, 55-59
  • K. BUCHHOLZ, B. G�RLITZ, F. HEYFELD, V. KASCHE, M. KIPER und W. van den Daele
    Technischer Fortschritt und fortschrittliche Technik. Wie nützlich ist die Bio-/Gen-Technologie"
    Bericht einer Studiengruppe des Vereins deutscher Wissenschaftler - im Auftrage des BMFT, 1985
  • V. KASCHE
    Research on enzyme and microbial technology in the Peoples Republic of China"
    Enzyme Microb. Technol., 4, 125-126 (1982)
  • V. KASCHE
    Biotechnische Entwicklungen zur Energiegewinnung und Stoffumwandlung"
    Mitteilungen des Verbandes deutscher Biologen Nr. 253 (1979) (Beilage zu: Naturwiss. Rundschauf, 3 (1979)
  • V. KASCHE, and J. KRAFT
    Framtidsforskningen och politiken"
    Liberal Debatt (Sweden), 22, 5-8 (1979)
  • V. KASCHE
    Molekulare Evolution und die Entstehung lebender Systeme - Zufall oder zwingende Notwendigkeit?"
    Jahrbuch der Wittheit zu Bremen. Hrsg.: Die Wittheit zu Bremen, Bremen (1976)
  • V. KASCHE
    Die fachsystematischen Aspekte bei der Biologielehrer - Ausbildung im Rahmen des Bremer Modells"
    Biologie und currikulare Forschung (D. Rodi, Hrsg.) Aulis Verlag Deubner & Co., Köln, 36-42 (1975)
  • V. KASCHE, H. AMNEUS, and K.J. JOHANSSON
    Den bl� elektonen (The blue electron)"
    Forskning och Framsteg (Sweden) 8, 30 (1973)


Books & book chapters

  • K. Buchholz, V. Kasche, U.T. Bornscheuer
    Biocatalysts and enzyme technology
    Wiley-VcH, Weinheim. 2005
  • K. Buchholz, V. Kasche
    Biokatalysatoren und Enzymtechnologie
    VCH, Weinheim, 1997


Patents

  • angemeldet (2002)
    Gentechnisch veränderte Penicillinamidase und Verfahren zu ihrer Herstellung
  • 5,326,698 (1994)
    Method of purification of penicillin amidase using phenylbutylamine-eupergit
  • GB 2 230 010 B � continuation (1990)
    Carrier comprising phenylbutylamine groups for purification or immobillisation of proteins
  • DE 39 09 018 A1 (1990)
    Protein-Reinigungsverfahren
  • DE 35 07 403 C 2 (1986)
    Enzymatische Semisynthese von ß-Lactamantibiotika
Kasche

News

New PhD position at ITB. Topic: Experimental and theoretical processing of biocatalytic cascade reactions.

New PhD position at ITB.

Topic: Experimental and theoretical processing of biocatalytic cascade reactions.

Complete offer information available here.

Edgar Gamero joins the ITB for his Master Thesis. Welcome!

Edgar Gamero joins the ITB for his Master Thesis. Welcome!

Edgar will work in the topic "Etablierung einer Hochzelldichtefermentation und eines Aktivitätstest zur Oxidation von Alkanen".

Good luck and welcome!

Dr. Martina Schedler has succesfully completed her PhD!

Martina Schedler has succesfully completed her PhD "Microbial degradation of crude oil at high pressure".

Congratulations!!

Aparna Nair joins the ITB for her Master's Project Work. Welcome!
Aparna Nair joins the ITB for her Master's Project Work. Welcome!

Aparna will work in the topic "Optimization of crude oil fractionation procedures for biodegradation analysis at high pressure conditions".

Good luck and welcome!